To investigate the role of activated, mutant EZH2 in melanoma biology we collected three melanoma cell lines harboring endogenous EZH2Y646 mutations (IGR1, C001 and MM386), a large panel of melanoma cell lines with wild-type (WT) EZH2 and non-transformed melanocytes (HEM) and dermal fibroblasts (HDF). The gene discussed is EZH2; the disease is melanoma.