DGKZ and colorectal neoplasm: The SW480 cell line is derived from a colorectal tumor and has characteristics of highly transformed tumors, including rapamycin resistance.30 DGKζ expression was silenced by interfering RNA (RNAi) with a previously validated sequence.22, 31 We determined total DGK activity in DGKζ-silenced cells and compared it with that of reducing DGKα, also expressed by this cell line.