TLR7, CTSS, and CTSC mRNA significant upregulation (p < 0.05) was found in the anterior lumbar spinal cord in ALS cases compared to control cases. IL10RA, TGFB1, and TGFB2 showed a tendency to increase without statistical significance. C1QTNF7 and TNFRSF1A mRNA were downregulated (p < 0.05). Here, CTSC is linked to amyotrophic lateral sclerosis.