Double-labelling immunofluorescence and confocal microscopy further supported colocalization of IL-10 and phosphorylated TDP-43 Ser409-410 in globular and skein-like inclusions of motor neurons in ALS, involving about 80% of TDP-43-immunoreactive inclusions (Figure 3). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.