In FAP7 Sanger sequencing identified only an APC c.3920 T > A (p.I1307K) mutation, and this would have probably stopped the analysis for this patient, as it is a FAP pathogenic mutation, albeit its clinical significance is still controversial [32, 33]; the NGS multiplex approach revealed a coexistent MUTYH c.536A > G (p.Y179C) mutation, which is reported as pathogenic and related to MAP syndrome [6, 17, 18]. Here, APC is linked to familial adenomatous polyposis 2.