Among the EBNA1 negative samples (n = 8), methylated DAPK was not detected, but one sample was positive only for P16 and another such sample was positive for ITGA9. A third EBNA1 negative sample was positive for ITGA9, RASSF1A, and P16. These samples might represent EBV-negative NPC. The gene discussed is RASSF1; the disease is nasopharyngeal carcinoma.