Western blot analysis was further performed on the 8 HCC pairs to confirm the protein expression of the candidate genes, including IFITM1, CHST4, TBX15, LRRC4, and NQO1. Compared with adjacent non-tumor tissue, we observed reduced protein expression of IFITM1 in 6 of 8 tumors, lower TBX15 levels in 7 of 8 tumors, and decreased CHST4 amounts in 5 of 8 tumors (Fig. 3c, d). This evidence concerns the gene CHST4 and hepatocellular carcinoma.