Using this approach, we confirmed that 7 genes showed altered promoter methylation in HCC, with SMAD6, IFITM1, LRRC4, CHST4, and TBX15 exhibiting promoter hyper-methylation, and CCL20 and NQO1 exhibiting promoter hypo-methylation. This evidence concerns the gene SMAD6 and hepatocellular carcinoma.