J. Wang et al., based on their previous study that hippocampal mAChR-1 participating in MARK signaling was involved in EA induced cumulative analgesia in neuropathic pain rats, observed in their present study that EA2W was closely related to the cumulative analgesia via intracellular ERK and p38 MARK signaling. The gene discussed is MARK1; the disease is neuropathic pain.