Similarly, in CP-4 with AML, FISH analysis identified the composition of double minutes as amplification of the 8q24 chromosome region containing MYC, while the region of amplification was further characterized by microarray as a 4.45 Mb segment encompassing the TRIB1, MYC and PVT1 genes (Figure 3a–3c). Here, TRIB1 is linked to acute myeloid leukemia.