To study the effect of exogenous HDGF on β-catenin signaling of CRC cells, nuclear and cytoplasmic HDGF, β-catenin, c-Myc, cyclin D1, MMP9 and phos-GSK-3β (Ser9) expression increased in LOVO cells in dose dependent manner. The gene discussed is CCND1; the disease is colorectal carcinoma.