Amongst the established CCA cell lines, HuCCT-1 (iCCA) and TFK-1 (eCCA) expressed LIFR and secreted LIF (Figure 2A, 2B), as confirmed by immunofluorescence in cultured cells (Figure 2C, 2D), therefore these cell lines were selected for subsequent in vitro experiments. The gene discussed is LIF; the disease is cholangiocarcinoma.