Through hierarchical clustering with distance matrix (Pearson), we analyzed the expression of a) proteins encoded by the 20 selected genes, b) several principal regulators of stemness and tumorigenicity in GSCs, c) reporters of the signaling pathways relevant for GBM such as: active CTNNB1 (ABC) (Wnt pathway), SHH and GLI1 (Hedgehog pathway), MTOR (MTOR pathway), DLL3 (Notch pathway) and STAT3 (Jak/stat pathway), d) proteins relevant for sub-classification of GBMs/GSCs at the protein level [26] and e) the reporters of growth factor signaling pathways (IGFBP5, PDGFB, EGFR and NF1). The gene discussed is GLI1; the disease is glioblastoma.