GCLC and hepatocellular carcinoma: In the orthotopic HCC model, TUDCA supplementation did not alter the expression levels of DEN-induced antioxidant response genes such as glutathione-S-transferase A1 (Gsta1), glutathione-S-transferase A2 (Gsta2), nuclear factor erythroid-derived 2 like 2 (Nfe2l2) and glutamate-cysteine ligase (Gclc; Supplementary Figure S2B).