We characterized the expression of genes that are closely associated with MDR, including MDR1/P-gp, GST-π, LRP, Bcl-2 and TS, before and after the modulation of HIF-1α expression in GC cells to elucidate the mechanism by which the HIF-1α-miR-27a pathway regulates the MDR of GC. This evidence concerns the gene BCL2 and gastric cancer.