TGFB1 and embryonal rhabdomyosarcoma: We examined the miR-411 cluster by reverse transcription-PCR (RT-PCR) using total RNA isolated from six low- and high-TGF-β1-expressing ARMS samples and eight paired ERMS samples, based on immunoreactive score (IRS) of TGF-β1 protein expression obtained as described previously.17 As anticipated, miR-411-5p expression differed significantly between low- and high-TGF-β1-expressing ARMS and ERMS tissues in three of six and five of eight lines, respectively, whereas miR-411-3p expression differed significantly in two of six and four of eight, respectively (Figure 1b).