In addition, in both of the sham and CHF myocytes, the pre‐treatment with anti‐TLR4 antibodies (5–10 μg/ml) significantly inhibited the binding of FITC‐LPS and OG‐HSP60 (Fig. 6B and C), suggesting that FITC‐LPS and OG‐HSP60 were bound to TLR4. This evidence concerns the gene HSPD1 and congestive heart failure.