Transcripts of DMD, the skeletal muscle cell-specific gene creatine kinase-muscle (CKM), and tropomyosin 2 (TPM2)29, a Ca2+ channel marker recognised in matured contractible muscle cells, were up-regulated at day 4 subsequent to over-expression of MyoD in Control-iPStet-MyoD and DMD-iPStet-MyoD cell-derived myocytes (denoted as Control- and DMD-Myocytes, respectively) (Fig. 2a). Here, TPM2 is linked to Duchenne muscular dystrophy.