NFKB1 and intervertebral disk degenerative disorder: To understand that suppression effects of TGF-βl in NF-κB-dependent inflammation are responsible for the protection against rabbit disc degeneration, we next used immunohistochemistry to detect the expressions of TGF-βl and NF-κB. Immunohistochemical analyses revealed that the expression of TGF-βl increased over time in the BMSCs transplantation group, and also the expression in these discs was significantly higher compared to those detected in the saline and SB431542 groups.