We exposed LT-CML stem cells to 5 μM GlySar or 5 μM cefadroxil for 30 min and used the highly sensitive Duolink in situ proximity ligation assay (D-PLA) to evaluate both phosphorylated Raptor-Ser863 and phosphorylated S6 ribosomal protein, which indicate mTORC1 activation26. Here, RPTOR is linked to chronic myelogenous leukemia, BCR-ABL1 positive.