In conclusion, we have demonstrated that the p53 rs1042522 GC genotype, but not the MDM2 rs2279744 or p21 rs1801270 genotypes, is associated with a reduced risk of RB invasion in patients with a lag time greater than 1 month or in patients who received no prior treatment before enucleation, suggesting an interaction of rs1042522 with lag time and pre-enucleation treatment status in RB invasion. The gene discussed is TP53; the disease is retinoblastoma.