To validate the regulatory relationship between NCOA1 and VEGFa in breast cancer cells, we further tested whether expression of NCOA1 in K1 and K2 Ncoa1 knockout cells could restore VEGFa expression and whether silencing NCOA1 expression in mouse and human breast cancer cells could down-regulate VEGFa expression. The gene discussed is NCOA1; the disease is breast carcinoma.