To test whether the increased susceptibility of BALB/c Foxp3.LuciDTR mice following Treg depletion could be reproduced in an independently generated Foxp3-DTR mouse strain, we evaluated BALB/c DEREG mice given DTx in the first 10 days of infection as previously described;23 however, this regimen resulted in appreciable weight loss and a high degree of morbidity so that experiments were discontinued (Figure 5n and data not shown). The gene discussed is FOXP3; the disease is infection.