Heightened pathology in BALB/c DEREG mice may also be explained by an increased overall dose of DTx given to these mice (1 μg vs. ∼480 ng for BALB/c Foxp3.LuciDTR), supporting the conclusions of a recent publication reporting pathology in DTx-treated wild-type mice following influenza infection.62, 63 Furthermore, near-complete depletion of Tregs in more susceptible C57BL/6 Foxp3.LuciDTR at days 14–26 every 2 days following infection with H. polygyrus resulted in weight loss but no change to parasite burden, despite increased antigen-specific Th2 responses in Treg-depleted mice. This evidence concerns the gene FOXP3 and infection.