In the MLN, depletion was also around 70% at steady state but decreased to around 45% following infection (Figure 7b), which was reflected by a smaller increase in proliferation of breakthrough Treg populations in Treg-depleted, infected mice (50% Ki67+) (Figure 7c) compared with BALB/c Luci-DTR mice (see Supplementary Figure S1B). Here, MKI67 is linked to infection.