MiR-200c was also upregulated in BM cells after exposed to B16 melanoma, CT-26 colon carcinoma, ID8 ovarian cancer or 4T1 breast tumor cell conditioned medium (TCCM, 25% v/v), especially to that of CT-26 and 4T1 (Fig 1C and 1D). This evidence concerns the gene DDX53 and melanoma.