To study the functional relationships between Treg-mediated systemic immune suppression, CP gateway activity and AD pathology, we cross-bred 5XFAD AD-Tg mice with Foxp3-diphtheria toxin (DTx) receptor (DTR+) mice, enabling transient conditional in vivo depletion of Foxp3+ Tregs31 in AD-Tg/DTR+ mice by administration of DTx (Supplementary Fig. 1c). Here, CP is linked to Alzheimer disease.