In order to examine whether increased motility of SW480 cells incubated in the presence of CM from senescent HPMCs was related to the development of the epithelial-mesenchymal transition (EMT), cancer cell morphology and the expression of E-cadherin, a marker of epithelial cells, and vimentin, a marker of mesenchymal cells [25], in cell lysates were analysed. This evidence concerns the gene CDH1 and cancer.