In the present study, we silenced SEMA4C by specific siRNA in NSCLC cells and found that SEMA4C knockdown worked just like the miR‐138 overexpression in NSCLC cells, and the ectopic expression of SEMA4C accordingly rescued the reversion effect of miR‐138 on EMT. Here, SEMA4C is linked to non-small cell lung carcinoma.