Eight weeks after reconstitution, we subjected the mice to asthma, sorted out WT (CD45.1+CD4+CD44hiCXCR5hiPD1hi) and Batf KO (CD45.2+ CD4+CD44hiCXCR5hiPD1hi) Tfh cells and intravenously transferred them into congenic C57BL6 (CD45.2+) or B6.SJL (CD45.1+) mice, respectively followed by intranasal challenge with Ova for 5 days. Here, CD4 is linked to asthma.