RUNX1 and amyotrophic lateral sclerosis: As noted above, Runx1 RNA expression was reported previously in various types of human muscle diseases including ALS and DMD and their respective mouse models tg-mSOD1 and mdx. Immunohistochemistry (IHC) analysis of gastrocnemius muscles by anti Runx1 antibodies (Ab) revealed no signal in untreated wild-type (WT) muscle (Fig 1A) and in developing muscle (S1A Fig), whereas it was readily detected in tg-mSOD1 muscles (Fig 1B) and in denervated muscles (see S2 Fig).