Because TGF-β1 signaling has been considered as a critical determinant of actin cytoskeletal dynamics that contributes to the structural changes in TM cells in the pathology of primary glaucoma [38], cytoskeletal arrangements induced by TGF-β1 (5 ng/mL) were assessed using actin polymerization assays. Here, TGFB1 is linked to Primary glaucoma.