Amplicons of the correct predicted sizes corresponding to the selected genes transcripts were successfully generated in all tested samples with the exception of one sample (PD-25a) that produced a shorter transcript of PARKIN (~160bp) which appears to be missing a 137bp fragment equivalent to the size of exon 7 (S3C Fig) The presence of transcripts for the selected genes in the tested PD samples, suggest that mutation(s) in the regulatory elements or deep intronic regions of these genes are more likely to be absent. The gene discussed is PRKN; the disease is Parkinson disease.