Here, we study the effect of T-614 on the RANKL, OPG, IL-17, and MMP-3 expression in a simulative inflammatory context by RASFs stimulated with IL-6 and conducted a comparative analysis of the antiarthritic of T-614 and MTX, the classic disease-modifying antirheumatic drugs (DMARDs) used for RA therapy. This evidence concerns the gene MMP3 and rheumatoid arthritis.