For example, it was shown that induction of NASH in rats leads to changes in expression and function of ATP-binding cassette (ABC) transporters responsible for the disposition of drugs, causing elevated expression of Abcc1-4, Abcb1, and Abcg2 transporters and mislocalization of Abcc2 and Abcb1 to the membrane thus rendering hepatocytes more susceptible to hepatocellular damage after administration of MTX [59]. This evidence concerns the gene ABCB1 and metabolic dysfunction-associated steatohepatitis.