Screening of various established breast tumor lines identified increased expression of MIEN1 in majority of the breast tumor lines (MCF10AT, MCF10CA1a, MCF10CA1d, MCF10CA1h, JIMT-1, BT-474, SKBR-3, MDA-MB231, T47D, MCF-7, MDA-MB436 and HCC-70) compared to the immortalized normal mammary epithelial cell line, MCF10A (Fig. 1c). This evidence concerns the gene MIEN1 and breast neoplasm.