In addition, several exhibited universally high expression in the PDX models, including AHNAK, BCL2L1, CDKN1A, CTNNB1, HRAS, RHOA, and YWHAZ. Similarly, evaluation of the 291 high-confidence driver (HCD) genes identified via pan-cancer analysis of TCGA datasets [27], and using the same criteria for analysis as described above, demonstrated that 199 HCD genes were expressed in the panel (Figure B in S2 Fig, S7 Table). This evidence concerns the gene RHOA and cancer.