Among the top 100 most highly phosphorylated peptides, we identified 86 peptides previously defined as activated peptides in pediatric ALL including cAMP responsive element binding protein 1 (CREB1), peptides derived from protein kinases related to the PI3K/Akt-signaling pathway including peptides related to phosphatidylinositol 3 kinases and ribosomal S6 kinases, and peptides derived from regulators of the cell cycle including checkpoint kinase 2 and retinoblastoma 1 [19]. The gene discussed is RB1; the disease is acute lymphoblastic leukemia.