To further confirm that whether it was the reduced TRB3 in tumour cells responsible for the tumour growth inhibition, B16-F10 cells stably expressing control-shRNA or TRB3-shRNA sequences were subcutaneously (s.c.)or intravenously (i.v.)injected into KK-Ay and C57 BL/6 mice. This evidence concerns the gene TRIB3 and neoplasm.