As shown in Fig. 2i, TRB3 expression in tumour nodules from TRB3 knockdown KK-Ay mice was much lower than that from control KK-Ay mice, suggesting that the higher insulin/IGF-1 in TRB3 knockdown KK-Ay mice cannot enhance TRB3 expression in the inoculated tumour cells as it does in the control KK-Ay mice. This evidence concerns the gene IGF1 and neoplasm.