We found that knocking down of ATG5, an essential autophagy gene, reverses the antitumour effect of TRB3 depletion in tumour cells as demonstrated by the 5-Ethynyl-2′-deoxyuridine (Edu) proliferation assay (Fig. 3h) and the transwell invasion assay (Fig. 3i). This evidence concerns the gene ATG5 and neoplasm.