TRIB3 and neoplasm: We found that knocking down of ATG5, an essential autophagy gene, reverses the antitumour effect of TRB3 depletion in tumour cells as demonstrated by the 5-Ethynyl-2′-deoxyuridine (Edu) proliferation assay (Fig. 3h) and the transwell invasion assay (Fig. 3i).