We also performed an in situ proximity ligation assay (ISPLA) in BORIS-positive ovarian cancer cells (OVCAR8) and human testis tissues, confirming that CTCF and BORIS proteins are co-localized in a chromatin context of both cancer and germ cells where the two proteins are co-expressed (Fig. 3c, d). The gene discussed is CTCF; the disease is ovarian cancer.