Further, results from EMSA (Fig. 3f; Fig. S5c in Additional file 5), ChIP-Re-ChIP (Fig. 1i; Fig. S2b in Additional file 2), co-immunoprecipitation (Fig. 3b), and ISPLA in both cancer and germ cells (Fig. 3c, d, i) support the co-occupancy of 2xCTSes by CTCF and BORIS. This evidence concerns the gene CTCF and cancer.