Exposure of cells to high D-glucose concentrations (HG) and culture in low-growth-factor medium, conditions that mimic diabetes and ischaemia in vitro (L-Glucose as an osmotic control; Cont), increases p75NTR mRNA and protein expression in both HUVECs (Fig. 1a,b) and HMVECs (Supplementary Fig. 2A,B). Here, NGFR is linked to diabetes mellitus.