10, 13 and 18 (data not shown), however, the same procedure showed strong CK18 staining in lung and HCC cancer cell line cells (Figure 2K and 2M) and in all of more than 20 of epithelial tumor cell lines including breast, pancreatic and colon cancers, etc. (data not shown) as well as in majority of CTCs detected in metastatic PDX (mPDX) mice [47], indicating that loss of detectable CK18 in CTCs is an unlikely consequence of the i•FISH® methodology. This evidence concerns the gene KRT18 and colonic neoplasm.