PTEN and glioblastoma: To further confirm the regulation of PTEN by miR-1908, the luciferase reporter containing the complimentary seed sequence of miR-1908 at the 3’-UTR region of PTEN mRNA was constructed (Fig. 6e), luciferase activity was detected at 48 h after the co-transfection of FLuci vector (3-UTR-PTEN wt FLuci vector or 3 -UTR-PTEN mut FLuci vector), miR-1908 mimic or NC mimic, and RLuci vector in glioblastoma cells.