Considering the previous results obtained, indicating that the toxin PS2Aa1 regulates positively ERK1/2 phosphorylation (MAPK pathway) and negatively AKT phosphorylation at serine 473 (AKT pathway), we decided to use inhibitors for both pathways in combination with PS2Aa1 to see if these pathways influence apoptosisinduction and caspases activity in PC3 cancer cell line. The gene discussed is MAPK3; the disease is cancer.