In contrast there was a decrease in OPN mRNA levels in the glioblastoma cell lines when cells were cultured under standard hypoxia (see Fig. 4: 24 h post irradiation), which was more pronounced the longer the cells were in culture (see Fig. 4: 48 h post irradiation) (p-values < 0.05). The gene discussed is SPP1; the disease is glioblastoma.