Next, to investigate the SPINK1-mediated global gene-expression profiles in colon adenocarcinoma, microarray analysis was performed using shSPINK1-1, shSPINK1-3, shSPINK1-6 and shSCRM cells on Agilent Human Whole Genome Oligo Expression Arrays (Agilent Technologies, Santa Clara, CA, USA). Here, SPINK1 is linked to colon adenocarcinoma.