Moreover, MT1F, MT1G, MT1X and MT2A gene expressions were significantly downregulated in CRC tissues.57, 58 In our current study, we found significant upregulation of the MT family members (MT1B, MT1X, MT1H, MT1L, MT1E, MT1M, MT2A and MT1G) on SPINK1 knockdown in colon cancer WiDr cells, which corroborates with other studies that downregulation of MTs is an early indicator of CRC progression. Here, SPINK1 is linked to malignant colon neoplasm.