Antisense to miR-221 suppressed the proliferative capacity, increased the amount of apoptosis, and sensitized the effects of gemcitabine in pancreatic cancer cells with concomitant up-regulation of PTEN, p27Kip1, p57Kip2, and PUMA, which are the tumor suppressors and the predicted targets of miR-221 [42,45,46]. This evidence concerns the gene BBC3 and familial pancreatic carcinoma.