Several functional studies have demonstrated that DAX-1 plays a critical role in Ewing sarcoma pathogenesis: DAX-1 knockdown impairs Ewing sarcoma cell proliferation, G1 cell arrest induction, inhibits anchorage independent growth of colonies in soft agar, and drastically inhibits growth of xenotransplanted tumor cells in immunodeficient mice (9, 25, 26). This evidence concerns the gene NR0B1 and neoplasm.