Thus, to determine if VSMC are sensitive to NF-κB-mediated RAGE signaling, we first quantified NF-κB activity via the measurement of the GFP signal in A7r5 cells stimulated with AGE-HSA, CML-HSA or PBS and transfected with a GFP-based gene reporter plasmid for NF-κB. This evidence concerns the gene ALB and chronic myelogenous leukemia, BCR-ABL1 positive.