Although there was no significant difference in the surface expression of TfR during infection in the siNT group, there was a moderate but significant reduction (1.5-fold, p = 0.0007, Fig 4H) in the TfR surface expression in infected cells compared to uninfected cells in the Rab35 knockdown group, indicating a requirement for Rab35 in maintaining the TfR surface levels during UPEC infection. This evidence concerns the gene RAB35 and infection.