Further highlighting the association between Rab35 and TfR1 during UPEC infection, we found that similar to Rab35 knock down cells, the reduced iron pool in TfR1 knockdown cells (Fig 4B) was also associated with reduced survival of UPEC at 24 and 48 h post infection (3.4 fold at 24 h, p = 0.02, Fig 4C). This evidence concerns the gene TFRC and infection.