NMDARs have well-established roles in traumatic brain injury and cerebral ischemia,30, 31 and a number of inhibitors targeting NMDAR-PSD95-nNOS complexes have been thoroughly investigated.32, 33, 34, 35, 36, 37 In the present study, we successfully established in vivo and in vitro NMDA injury models. The gene discussed is NOS1; the disease is brain ischemia.