DIRAS3 and ovarian carcinoma: Necrostatin-1 (Nec-1), a RIP1-specific inhibitor, has been reported to block RIP1/RIP3-mediated necroptosis.30, 31 Treatment with Nec-1 significantly decreased the ARHI-induced loss of cell viability in SKOv3-ARHI and HEY-ARHI ovarian cancer cells after treatment with DOX (Figure 4d and Supplementary Figure S7C) and reduced the induction of necrosis after ARHI re-expression (Supplementary Figure S8).