One factor that can contribute to the induction of necrosis is the excessive production of reactive oxygen species (ROS) that leads to oxidative stress and damage of intracellular organelles.28, 29 To determine whether re-expression of ARHI increases ROS production, SKOv3-ARHI ovarian cancer cells were treated with DOX; ROS were then measured in cell lysates using Cell Biolabs OXiSelect ROS kit (San Diego, CA, USA). Here, DIRAS3 is linked to ovarian cancer.