DIRAS3 and ovarian cancer: Expression of ARHI is downregulated in ovarian cancers by multiple mechanisms.17, 18, 32, 33 Re-expression of ARHI drives autophagy by interacting at several points in the development of autophagolysosomes.15, 16, 20 Persistent re-expression of ARHI produces loss of clonogenic potential of ovarian cancer cells within 3 days in cell culture.16 Autophagy-associated ARHI-induced cell death is associated with modest G1 cell-cycle arrest and necrosis, but neither apoptosis nor senescence.