Initially, our data demonstrated that KAI1- and p27- were the two markers among 7 previously identified independent markers that showed the most significant difference in expression level between metastatic melanomas (95.24% vs. 78.10%) and primary melanomas (66.21% vs. 50.34%, P < 10E-4) (Table 2). The gene discussed is CDKN1B; the disease is metastatic melanoma.