AFAP1 and nasopharyngeal carcinoma: We next assessed AFAP1-AS1 expression in paraffin embedded NPC samples via in situ hybridization and found that AFAP1-AS1 expression was upregulated in NPC samples when compared with non-tumor NPE samples (69.64%, 78/112 vs. 20%, 2/10; p < 0.001; Figure 2F).