We confirmed these results via Western blotting analysis of tumor cells expressing AFAP1-AS1 siRNA, and found that RhoA, Rac2, Rab10, Rab11a, Rhogdi and Pfn1 were significantly upregulated, but RhoC, Rab11b and Lasp1 were significantly downregulated in NPC cell lines (Figure 8). The gene discussed is AFAP1; the disease is nasopharyngeal carcinoma.