AFAP1 and neoplasm: We confirmed these results via Western blotting analysis of tumor cells expressing AFAP1-AS1 siRNA, and found that RhoA, Rac2, Rab10, Rab11a, Rhogdi and Pfn1 were significantly upregulated, but RhoC, Rab11b and Lasp1 were significantly downregulated in NPC cell lines (Figure 8).